A woman, who had been married for 4 years without pregnancy, went to hospital for primary infertility in November 2018. She had regular menstruation and underwent hysterosalpingography which indicated that both fallopian tubes were unobstructed. After cytogenetic examination, the clinical diagnosis was: primary infertility, oligoasthenospermia, chromosome abnormality 46, XY, t (14; 16)(q24; P13.3). The husband was a chromosome balanced translocation carrier, which was inherited from his mother. After genetic counseling, the doctor recommended the couple to try IVF. Both partners had no abnormalities in the pre-ART examination. After the informed consent was signed, ICSI was performed after ovulation induction. The embryos were cultured until the fifth day, and nine embryos were selected for PGT-SR. 5ml of peripheral blood was extracted from the couple, and haplotype analysis was used to distinguish embryos of balanced translocation. Finally, four embryos were completely normal diploid embryos. After morphological evaluation, the embryo rated 4BB was selected for implantation. Amniocentesis was performed at 18 weeks of gestation and the prenatal diagnosis results were consistent with the embryo test results. The fetus had no chromosome abnormalities. On December 22, 2019, she gave birth to a male baby weighing 6.3 kg at 39 weeks and 2 days of pregnancy.